The invention discloses a universal high-and-low temperature automatic sample conveying device conveying kit. The universal high-and-low temperature automatic sample conveying device conveying kit comprises a feeding rotation and positioning groove, a discharging rotation and positioning groove, a material transfer tray and a piston head. Different limiting modules are correspondingly arranged onthe feeding rotation and positioning groove, the discharging rotation and positioning groove, the material transfer tray and the piston head for the demand of the test of chips with different packaging sizes. The universal high-and-low temperature automatic sample conveying device conveying kit can be suitable for the chips with different packaging sizes, the manufacturing cost of the conveying kit is reduced, and the utilization rate of the machine is increased.