The invention provides full-automatic AOI (automatic optical inspection) backlight detection device. The device comprises an AOI backlight detection mechanism, a manipulator feeding mechanism, a firstconveying line and a second conveying line, wherein the AOI backlight detection mechanism comprises a rack, a four-station turntable arranged in the rack, an identification code reader, a cleaning assembly, a CCD detection assembly, and four groups of lighting fixtures which are correspondingly arranged on the four-station turntable; the identification code reader, the cleaning assembly, the CCDdetection assembly and the manipulator feeding mechanism are sequentially arranged on the four stations in the rotation direction of the four-station turntable; the rack comprises an inlet positionedon a station where the identification code reader is positioned and an outlet positioned on a station where the manipulator blanking mechanism is positioned; the first conveying line, the manipulatorfeeding mechanism and the second conveying line are arranged on one side of the outlet in a straight line form;and the first conveying line and the second conveying line are used for shunting liquid crystal panels with qualified and unqualified detection result.