The invention discloses a plug-and-play two-way temperature acquisition circuit, and belongs to the technical field of temperature acquisition. The plug-and-play two-way temperature acquisition circuit comprises a thermistor Rx, a thermistor socket J, a first resistor R1 and a second resistor R2, wherein the thermistor Rx is in plug-in connection with the thermistor socket J; the first pin of thethermistor socket J is connected with one end of the first resistor R1 to jointly form an AD1 acquisition end; the other end of the first resistor R1 is connected with a direct-current power supply VCC; the second pin of the thermistor socket J is connected with one end of the second resistor R2 to jointly form an AD2 acquisition end; and the other end of the second resistor R2 is connected to theground. The circuit has the advantage that the two-way acquisition structure is equivalent to differential acquisition, and interference on two acquisition branches can counteract each other, so thatinterference can be avoided; each voltage stabilizing diode can stabilize the voltage of the circuit in a controllable range, so that the circuit can be well protected.