The invention relates to the field of liquid-based cell flake production instruments, and specifically relates to a tripping and separating mechanism for liquid-based cell flake production. The tripping and separating mechanism comprises a lifting slide glass clamping mechanical arm and a tripping workbench, wherein the lifting slide glass clamping mechanical arm comprises a lifting driving mechanism, a rotary driving mechanism, a rotary shaft and a clamping jaw; a lifting sliding block driven to move on a vertical direction is arranged on the lifting driving mechanism; a bearing seat is arranged on the lifting slide block, a shaft sleeve driven by the rotary driving mechanism to rotate is arranged on the rotary driving mechanism and is fixedly sleeved on the rotary shaft; the tripping workbench comprises a workbench body, a flake production frame overturning device and a flake production frame recycling device; the flake production frame overturning device comprises a flake productionframe fixed seat and an overturning driving device. The flake production fixed seat capable of overturning is arranged on the tripping and separating mechanism so as to convert the flake production frame among different postures, the lifting slide glass clamping mechanism arm is conveniently to rotate and trip the flake production frame, separate the flake production frame and separate the slideglass.