The invention discloses a high-stability second near-infrared small-molecule fluorescence probe and a production method and application thereof. The novel high-stability second near-infrared small-molecule fluorescence probe is designed and synthesized, has strong tissue penetration capability, is almost free from interference of autofluorescence, and can achieve living second near-infrared fluorescent and photoacoustic imaging with high temporal-spatial resolution. The target probe can conduct radioactive 125I isotope labeling by means of a chloramine T method to achieve SPECT/CT imaging of small living animals. Meanwhile, the target probe has good active targeting to tumor tissues, a photothermal therapy effect on tumors is significant, and diagnosis and treatment integration of the tumors is achieved.