The invention discloses a torque force adjusting and locking structure of a torque wrench. The structure comprises a main pipe with a pipe hole communicating the two ends of the main pipe, a torque force adjusting set, a limiting guide sleeve, a rotating set and a locking screw piece; the torque force adjusting set is arranged in the pipe hole; the torque force adjusting set comprises a screw guiding piece, a screw rod, a rotating piece and a spring, wherein the screw rod, the rotating piece and the spring are assembled with the screw guiding piece in a screwed mode; the screw rod is providedwith a locking bolt penetrating out of the rotating piece; the limiting guiding sleeve is arranged on the screw rod in the pipe hole in a sleeving manner; the rotating set comprises a rotating pipe and a fixing screw piece; the rotating pipe is internally provided with an inner thread section and a sleeving section which are located at the two ends; the rotating pipe drives the rotating piece andthe screw rod combined with the rotating piece to spirally rotate relative to the screw guiding piece and axially move forwards and backwards in the pipe hole to adjust the torque force; the locking screw piece and the locking bolt penetrating out of the rotating piece are assembled in a screwed mode; and the locking screw piece pushes a plurality of locking plug pins to force the limiting guidingsleeve to be fixed, so that the torque wrench is locked and the torque force can not be adjusted.