Invention Publication
EP3146046A1 HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS
审中-公开
HAPLOIDOMBESTIMMUNG DURCH DIGITALISIERTE TRANSPOSONE
- Patent Title: HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS
- Patent Title (中): HAPLOIDOMBESTIMMUNG DURCH DIGITALISIERTE TRANSPOSONE
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Application No.: EP15727787.2Application Date: 2015-05-21
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Publication No.: EP3146046A1Publication Date: 2017-03-29
- Inventor: XI, Lei , WANG, Xiaohui , UNGER, Marc , RUFF, David
- Applicant: Fluidigm Corporation
- Applicant Address: 7000 Shoreline Court, Suite 100 South San Francisco, CA 94080 US
- Assignee: Fluidigm Corporation
- Current Assignee: Fluidigm Corporation
- Current Assignee Address: 7000 Shoreline Court, Suite 100 South San Francisco, CA 94080 US
- Agency: Thom, Russell
- Priority: US201462002733P 20140523
- International Announcement: WO2015179706 20151126
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C12Q1/68
Abstract:
In certain embodiments, the present invention provides a way of “digitally” marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.
Public/Granted literature
- EP3146046B1 HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS Public/Granted day:2020-03-11
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