Invention Grant
- Patent Title: Electric pulse generator for electroporator and electroporator apparatus provided with electric pulse generator
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Application No.: US15370918Application Date: 2016-12-06
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Publication No.: US10004899B2Publication Date: 2018-06-26
- Inventor: Yasuhiko Hayakawa , Kiyoshi Hayakawa , Masahiko Sugiyama , Dai Ayusawa , Kensuke Miki
- Applicant: NEPA GENE CO., LTD.
- Applicant Address: JP Ichikawa-shi
- Assignee: NEPA GENE CO., LTD.
- Current Assignee: NEPA GENE CO., LTD.
- Current Assignee Address: JP Ichikawa-shi
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- Priority: JP2014-152730 20140728
- Main IPC: A61N1/32
- IPC: A61N1/32 ; A61N1/30 ; C12N15/09 ; H03K3/00 ; C12M1/42

Abstract:
An electric pulse generator for electroporator includes a poring pulse generator, and a transfer pulse generator. The poring generator includes an n-stage Cockroft-Walton circuit, and a first circuit branched from a branching point of wiring on output side of the Cockroft-Walton, the first circuit includes a switching switch that is turned off in high-voltage mode and turned on in low-voltage mode, a voltage value for switching between the modes is in 200 to 1400 V, the first circuit includes a second circuit in which m2 series of m1 series-connected capacitors are connected in parallel, the switch and second circuit are connected in series, and first wiring on output side of the second circuit is merged with second wiring on output side of the Cockroft-Walton on output side of the branching point, and a withstand voltage of the Cockroft-Walton is a value in 1500 to 5000 V in the high-voltage mode.
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