A device for the culturing and/or isolation and/or detection and/or identification and/or counting of at least one target microorganism in a sample liable to contain it, which includes a support and a nutrient medium; the support including: a hydrophilic fibrous substrate, at least one porous layer in contact with one of the faces of the fibrous substrate, including a pigment or a mixture of pigments and at least one binder, the pigment having a size of less than 5 μm and the amount of the pigment or the mixture of pigments being between 50 and 97% by dry weight, with respect to the dry weight of the porous layer; the nutrient medium being included in the fibrous substrate. Also relates to the use and to the process for the manufacture of the device.