A system for on-demand cloning of virtual machines (VMs) includes a virtual server to host a number of VMs, the virtual server including at least one master VM. The system also includes a Web server to authenticate a user in response to a request for online access to a new VM on the virtual server. In addition, the system includes a cloning module, in communication with the Web server and the virtual server, to automatically clone the master VM to create a unique VM clone for the user on the virtual server responsive to the request.