Invention Grant
- Patent Title: Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase
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Application No.: US15268424Application Date: 2016-09-16
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Publication No.: US10011856B2Publication Date: 2018-07-03
- Inventor: Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
- Applicant: Genentech, Inc.
- Applicant Address: US CA South San Francisco
- Assignee: Genentech, Inc.
- Current Assignee: Genentech, Inc.
- Current Assignee Address: US CA South San Francisco
- Agency: Morrison & Foerster LLP
- Main IPC: C12N15/63
- IPC: C12N15/63 ; C12N15/85 ; A61K48/00 ; C07H21/04 ; C12P21/00 ; C12N15/113 ; C12N15/11 ; C12N1/38
Abstract:
The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
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