Invention Grant
- Patent Title: Fluorescence sensor for target analysis, kit for target analysis, and target analysis method using same
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Application No.: US14906897Application Date: 2014-06-27
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Publication No.: US10012631B2Publication Date: 2018-07-03
- Inventor: Naoto Kaneko , Ikuo Shiratori , Katsunori Horii , Jou Akitomi , Iwao Waga
- Applicant: NEC Solution Innovators, Ltd.
- Applicant Address: JP Tokyo
- Assignee: NEC Solution Innovators, Ltd.
- Current Assignee: NEC Solution Innovators, Ltd.
- Current Assignee Address: JP Tokyo
- Agency: Wilmer Cutler Pickering Hale and Dorr LLP
- Priority: JP2013-152476 20130723
- International Application: PCT/JP2014/067125 WO 20140627
- International Announcement: WO2015/012059 WO 20150129
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/04 ; C12N15/115 ; G01N33/542 ; C12Q1/6825 ; C12N15/11

Abstract:
The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same.The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.
Public/Granted literature
- US20160146772A1 FLUORESCENSE SENSOR FOR TARGET ANALYSIS, KIT FOR TARGET ANALYSIS, AND TARGET ANALYSIS METHOD USING SAME Public/Granted day:2016-05-26
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