An image frame motion estimation device and image frame motion estimation method using the same include performing first sampling for generating a first sample source block by performing first-type sampling on pixels of a source block; performing second sampling for generating a first sample reference block by performing first-type sampling on pixels of a reference block; determining a first matching region by comparing pixel values of the first sample source block and the first sample reference block; and determining a second matching region corresponding to the source block by comparing pixel values of a plurality of regions adjacent to the first matching region and the source block. Where one pixel is sampled for each block constituted by N pixels (N is a natural number) in width and M pixels (M is a natural number) in height and the sampled pixels are projected in a horizontal direction, the first-type sampling causes at least two pixels to be sampled for every M pixels or where one pixel is sampled for each block constituted by N pixels (N is a natural number) in width and M pixels (M is a natural number) in height and the sampled pixels are projected in a vertical direction, the first-type sampling causes at least two pixels to be sampled for every N pixels.