The present application discloses an atomization assembly, an electronic cigarette with a limited lifetime and a method of limiting a lifetime of the electronic cigarette. The atomization assembly includes an interface circuit detachably connected with the battery assembly, an atomization circuit and a main circuit. The atomization circuit and the main circuit are both connected with the interface circuit. The main circuit is used to count the puff number according to actual conducting times of the atomization circuit, after electrically communicated with the battery assembly via the interface circuit, so that the electronic cigarette controls the atomizing circuit to be disconnected and stopped from working when the puff number reaches a preset value. A technical effect is achieved that exactly counting the puff number and effectively ensuring the consistency between the available puff number and available amount of tobacco tar.