Invention Grant
- Patent Title: Purification method of virus-like particles
-
Application No.: US14904364Application Date: 2014-05-21
-
Publication No.: US10023844B2Publication Date: 2018-07-17
- Inventor: Masahiro Okada , Akiko Mukai , Tomonori Nishino , Daisuke Arinobu , Hiroyuki Ito , Mamoru Satoh
- Applicant: UMN PHARMA INC. , Timo Vesikari , Vesna Blazevic
- Applicant Address: JP Akita-shi, Akita FI Tampere FI Tampere
- Assignee: UMN PHARMA INC.,Timo Vesikari,Vesna Blazevic
- Current Assignee: UMN PHARMA INC.,Timo Vesikari,Vesna Blazevic
- Current Assignee Address: JP Akita-shi, Akita FI Tampere FI Tampere
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2013-146240 20130712
- International Application: PCT/JP2014/063423 WO 20140521
- International Announcement: WO2015/004996 WO 20150115
- Main IPC: C12N7/02
- IPC: C12N7/02 ; C12N7/00

Abstract:
The present invention aims at establishing a novel means for purifying norovirus VLPs. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM.
Public/Granted literature
- US20160168543A1 PURIFICATION METHOD FOR VIRUS-LIKE PARTICLES Public/Granted day:2016-06-16
Information query