An assembly and method to repeatedly set and isolate multiple sections along a zone of interest in a single downhole trip is disclosed. The assembly includes an outer pipe and an inner pipe adapted to telescope along the outer pipe. After a first section of a zone of interest is isolated, the inner pipe may be telescoped up along the outer pipe, and then set to isolate a second section above the first section. This process may be repeated as desired to stimulate and/or test each desired section along a zone of interest. Once the inner pipe is completely telescoped inside the outer pipe, the inner pipe may be disconnected from the outer pipe via use of a disconnect assembly.