This liquid handling device is provided with a first chip that comprises a first substrate, a first film, a first flow path, a second flow path and a partition wall. A diaphragm portion of the first film is over the first flow path, the second flow path and the partition wall, and the center thereof is arranged over the first flow path. The first chip comprises a discharge hole or a discharge flow path which, in the liquid flow direction in the first flow path, opens into the first flow path between the partition wall and the center of the diaphragm and allows communication between the inside and outside of the first flow path.