The present invention discloses a gate driving method of a pixel transistor and a gate drive circuit, as well as a display device including the gate drive circuit, which falls within the field of display technology. The method comprises the steps of: a gate drive circuit outputting a preset first voltage to a gate driving line of a pixel row prior to a transistor turn-on time of the pixel row, wherein the first voltage is greater than a transistor turn-off voltage; and the gate drive circuit outputting a transistor turn-on voltage to the gate driving line of the pixel row when it reaches the transistor turn-on time. Use of the present invention can improve the accuracy of pixel display.