A method for transmitting a demodulation reference signal (DMRS) for a physical uplink shared channel (PUSCH), by a user equipment (UE), in a wireless communication system. The UE generates first, second, and third DMRS sequences, which are associated with first, second, and third layers respectively, by applying first, second, and third cyclic shifts to the first, second, and third DMRS sequences respectively. The UE transmits the first, second, and third DMRS sequences to a base station, wherein the first, second, and third cyclic shifts are determined based on first, second, and third cyclic shift values respectively. The first and second cyclic shift values are separated from each other by a maximum separation value, which is determined based on a total number of cyclic shifts. The third cyclic shift value is determined by increasing cyclically by a median separation value between the first and second cyclic shift values.