A reusable portable decontamination system is provided for a transportation asset. The decontamination system includes a plurality of insulated panels detachably secured together to form an enclosure for the transportation asset and other equipment. The decontamination system also includes a humidifying module in fluid communication with an interior of the enclosure to raise a temperature and a humidity of the interior of the enclosure to a predetermined temperature level and a predetermined humidity level for a timed duration. Additionally, the decontamination system includes a dehumidifying module in fluid communication with the interior of the enclosure to lower the temperature and the humidity of the interior of the enclosure from the predetermined temperature level and the predetermined humidity level to ambient levels. A storage and decontamination module is also presented, to decontaminate one or more components of a transportation asset.