Invention Grant
- Patent Title: Method for identifying antigen-specific antibodies in primate
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Application No.: US15208850Application Date: 2016-07-13
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Publication No.: US10042975B2Publication Date: 2018-08-07
- Inventor: Guo-liang Yu
- Applicant: Qyuns Therapeutics Co., Ltd.
- Applicant Address: CN Taizhou
- Assignee: Qyuns Therapeutics Co., Ltd.
- Current Assignee: Qyuns Therapeutics Co., Ltd.
- Current Assignee Address: CN Taizhou
- Agency: Jun He Law Offices P.C.
- Agent James J. Zhu
- Main IPC: G06F19/22
- IPC: G06F19/22 ; C07K16/18 ; C07K16/06 ; C40B30/04 ; C07K16/28 ; C07K14/725 ; G01N33/50

Abstract:
Provided are methods for identifying antibodies that bind to an antigen in primates. In certain embodiments, the methods comprise: sequencing the cDNA prepared from a combined population of antibody-producing B-cells to obtain a plurality of antibody heavy chain sequences and a plurality of antibody light chain sequences; grouping the obtained antibody heavy chain sequences into heavy chain groups based on their CDR3 sequences; grouping the obtained antibody light chain sequences into light chain groups based on their CDR3 sequences; pairing the heavy chain groups with the light chain groups based on the number of antibody heavy chain or light chain sequences in each group; pairing one antibody heavy chain sequence and one light chain sequence in each paired heavy chain group and light chain group; and testing candidate antibodies comprising the paired heavy chain and light chain sequences for binding to the antigen.
Public/Granted literature
- US20170146551A1 METHOD FOR GENERATING PRIMATE ANTIBODIES AND T CELL RECEPTORS Public/Granted day:2017-05-25
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