A gas cylinder cart includes a frame, a first control assembly composed of a first control panel wall and a first gas distribution assembly, a second control assembly composed of a second control panel wall and a second gas distribution assembly, and a central manifold connected to each of the first and second control assemblies. The central manifold includes a valve assembly allowing for connection of both the first and second control assemblies to gas cylinders, connection of only one of the first and second control assemblies to gas cylinders, or connection of the first and/or second control assemblies to remote gas sources. Inclusion of the first and second control assemblies within the gas cylinder cart allows for use of the gas cylinder cart in conjunction with different systems that might require different control assemblies.