Invention Grant
- Patent Title: High pH protein refolding methods
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Application No.: US14766848Application Date: 2014-02-11
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Publication No.: US10065987B2Publication Date: 2018-09-04
- Inventor: Cristopher Hollander , Benjamin C. Blum
- Applicant: BRISTOL-MYERS SQUIBB COMPANY
- Applicant Address: US NJ Princeton
- Assignee: BRISTOL-MYERS SQUIBB COMPANY
- Current Assignee: BRISTOL-MYERS SQUIBB COMPANY
- Current Assignee Address: US NJ Princeton
- Agency: Nelson Mullins Riley & Scarborough LLP
- Agent Jane E. Remillard, Esq.; Christopher L. Frank
- International Application: PCT/US2014/015702 WO 20140211
- International Announcement: WO2014/126884 WO 20140821
- Main IPC: C07K1/113
- IPC: C07K1/113 ; C07K14/78 ; C12P21/02
Abstract:
Provided herein are methods for refolding denatured protein (e.g., from inclusion bodies) that do not require the use of a denaturing agent. Exemplary methods use a high pH for solubilizing denatured protein, followed by a decrease in pH for refolding the proteins.
Public/Granted literature
- US20160002287A1 HIGH PH PROTEIN REFOLDING METHODS Public/Granted day:2016-01-07
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