Invention Grant
- Patent Title: Foreign substance introduction device and method of producing cells with introduced foreign substance
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Application No.: US14650560Application Date: 2013-12-12
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Publication No.: US10066199B2Publication Date: 2018-09-04
- Inventor: Akira Mizuno , Rika Numano , Hirofumi Kurita
- Applicant: NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY
- Applicant Address: JP
- Assignee: NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY
- Current Assignee: NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY
- Current Assignee Address: JP
- Agency: Day Pitney LLP
- Priority: JP2012-271810 20121212
- International Application: PCT/JP2013/083374 WO 20131212
- International Announcement: WO2014/092164 WO 20140619
- Main IPC: C12M1/42
- IPC: C12M1/42 ; C12N15/87 ; C12N13/00

Abstract:
An exogenous substance transfer apparatus that introduces an exogenous substance into a small amount of cells by electrical action and a method for manufacturing exogenous substance-bearing cells at low cost are provided.In the introducing an exogenous substance like a gene into a cell, a cell suspension liquid that contains the cell and the exogenous substance is supplied to a container through an opening portion in an amount that is prepared such that a liquid droplet that is formed will not come into contact with a pair of electrodes simultaneously. The liquid droplet is formed in an oil stored in the container, without the cell suspension liquid mixing with the oil. When a direct current voltage is supplied to the electrodes from a power supply, the liquid droplet moves there-between, and the exogenous substance is introduced into the cell within the liquid droplet by electrical action.
Public/Granted literature
- US20150368604A1 FOREIGN SUBSTANCE INTRODUCTION DEVICE AND METHOD OF PRODUCING CELLS WITH INTRODUCED FOREIGN SUBSTANCE Public/Granted day:2015-12-24
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