Foreign substance introduction device and method of producing cells with introduced foreign substance
Abstract:
An exogenous substance transfer apparatus that introduces an exogenous substance into a small amount of cells by electrical action and a method for manufacturing exogenous substance-bearing cells at low cost are provided.In the introducing an exogenous substance like a gene into a cell, a cell suspension liquid that contains the cell and the exogenous substance is supplied to a container through an opening portion in an amount that is prepared such that a liquid droplet that is formed will not come into contact with a pair of electrodes simultaneously. The liquid droplet is formed in an oil stored in the container, without the cell suspension liquid mixing with the oil. When a direct current voltage is supplied to the electrodes from a power supply, the liquid droplet moves there-between, and the exogenous substance is introduced into the cell within the liquid droplet by electrical action.
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