The invention relates to a device for simultaneous fluorescence contrasting effect in transmitted light and reflected light, having a reflected light optical path for focusing of the excitation light via a lens onto a sample, having a fluorescence signal, which extends from the sample and is directed onto the same lens, having a dichroite, an emission filter, and a detection unit for the purpose of separating the excitation light from the fluorescence signal and for detection, having a luminescent layer behind the sample and a diaphragm for partial coverage of the excitation optical path between the sample and the luminescent layer, whereby a part of the excitation optical path, which impinges onto the luminescent layer, emits light, which irradiates the sample past the diaphragm by forming an oblique transmitted light illumination.