A touch unit and a manufacturing method thereof and a touch substrate are provided. The touch unit includes a first touch electrode, a second touch electrode and a conductive branch, the second touch electrode includes a first sub-electrode, a second sub-electrode and a conductive bridge wire, the first sub-electrode is located at one side of the first touch electrode and the second sub-electrode is located at the other side of the first touch electrode, the conductive bridge wire strides over the first touch electrode and two ends of the conductive bridge wire are connected with the first sub-electrode and the second sub-electrode respectively; and the conductive branch is connected with the second touch electrode and insulated from the first touch electrode, and an orthogonal projection of the conductive branch on a plane the first touch electrode at least partially located falls in a region corresponding to the first touch electrode.