A calibration method for a digital-to-analog converter (DAC) is disclosed. The DAC is applied to a successive approximation analog-to-digital converter (SA ADC) and includes a first capacitor, multiple second capacitors and a bridge capacitor. The method includes the steps of: (a) controlling voltages at two input terminals of a comparator of the SA ADC to be equal; (b) changing a voltage at a first terminal of the first capacitor; (b) obtaining a first output of the SA ADC; (d) after obtaining the first output, controlling voltages at the two input terminals of the comparator to be equal; (e) changing voltages at multiple first terminals of the second capacitors; (f) obtaining a second output of the SA ADC; and (g) calibrating the DAC according to the first output and the second output.