Invention Grant
- Patent Title: Method for removal of triglycerides in lipoproteins other than low-density lipoproteins
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Application No.: US14395449Application Date: 2013-04-19
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Publication No.: US10077286B2Publication Date: 2018-09-18
- Inventor: Motoko Ohta
- Applicant: DENKA SEIKEN CO., LTD.
- Applicant Address: JP Tokyo
- Assignee: DENKA SEIKEN CO., LTD.
- Current Assignee: DENKA SEIKEN CO., LTD.
- Current Assignee Address: JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2012-097161 20120420
- International Application: PCT/JP2013/061672 WO 20130419
- International Announcement: WO2013/157642 WO 20131024
- Main IPC: C07K1/14
- IPC: C07K1/14 ; G01N33/92 ; G01N33/68 ; C12Q1/26 ; C12Q1/44 ; C12Q1/48 ; C12Q1/61

Abstract:
Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
Public/Granted literature
- US20150132834A1 METHOD FOR REMOVAL OF TRIGLYCERIDES IN LIPOPROTEINS OTHER THAN LOW-DENSITY LIPOPROTEINS Public/Granted day:2015-05-14
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