Invention Grant
- Patent Title: Cell-free protein expression using rolling circle amplification product
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Application No.: US15145838Application Date: 2016-05-04
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Publication No.: US10077459B2Publication Date: 2018-09-18
- Inventor: John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
- Applicant: General Electric Company
- Applicant Address: US NY Schenectady
- Assignee: GENERAL ELECTRIC COMPANY
- Current Assignee: GENERAL ELECTRIC COMPANY
- Current Assignee Address: US NY Schenectady
- Agency: GE Global Patent Operation
- Agent Pabitra Chakrabarti
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C12N15/63 ; C12N15/67
Abstract:
Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
Public/Granted literature
- US20170321239A1 CELL-FREE PROTEIN EXPRESSION USING ROLLING CIRCLE AMPLIFICATION PRODUCT Public/Granted day:2017-11-09
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