A fluid sample collection card assembly in one embodiment includes a case including a tray assembly receiving cavity accessible through a mouth portion of the case, and a tray assembly, the tray assembly including an absorbent layer supported by a base portion, the base portion including (i) a tray portion configured to be removably received within the tray assembly receiving cavity and (ii) a faceplate portion configured to at least substantially close the mouth when the tray portion is received within the tray assembly receiving cavity.