Systems and methods for blue light adjustment with a wearable display system are provided. Embodiments of the systems and methods for blue light adjustment can include receiving an initial eye image obtained by an image capture device; adjusting a level of blue light exposed to an eye associated with the initial eye image; receiving an adjustment eye image of the eye exposed to the adjusted level of blue light; detecting a change in a pupillary response of the adjustment eye image relative to the initial eye image; determining that the detected change in the pupillary response passes a biometric application threshold; and utilizing eye images or the detected change in the pupillary response for a biometric application.