A TFT substrate, a display device and a manufacturing method are disclosed. The TFT substrate includes a substrate and a first TFT structure and a second TFT structure formed on the substrate. The first TFT structure includes a first gate pattern and a first semiconductor pattern. The first semiconductor pattern is divided into a first channel region, and a first doping region and a second doping region located at two sides of the first channel region. The first channel region is disposed corresponding to the first gate pattern to form a first conductive channel under the function of first gate pattern. The first doping region is extended inside the second TFT structure as a second gate pattern of the second TFT structure. The present invention uses doping drain of a switching TFT as gate of a driving TFT to save layout space, and beneficial for realization of higher PPI.