A process for realising a device (1) for collecting and transferring samples for molecular biology, the device including at least: a support body (2) having at least a first portion (2a), and a plurality of fibers (6) attached and arranged on the first portion (2a) of the support body (2) by flocking, such as to define a flocked collecting portion (3) destined to collect, on the said collecting portion, (3) a quantity of the sample for molecular biology, the process including at least a step of pre-treating the plurality of fibers (6) with a surfactant, the step of pre-treating the plurality of fibers (6) with a surfactant being performed after application of the fibers on the support body (2) by flocking and/or before application of the fibers (6) on the body (2) by flocking and/or wherein the surfactant is added to the fiber (6) during production of the fiber (6).