The present invention discloses a centrifuge filtration tube comprising a centrifuge tube (2) and a centrifuge tube (1) housed in the centrifuge tube (2). One or more constant-volume vents (7) and multiple pores (4) are provided on a sidewall of the centrifuge tube (1). The one or more constant-volume vents (7) are located above the pores (4). The pores (4) are covered with a filtering membrane (3). The disclosed centrifuge filtration tube has the advantages of being simple, easy to use, highly efficient, and inexpensive, which can save large amounts of labor and time in analyses of large number of samples.