Invention Grant
- Patent Title: Regulated switch for gene expression
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Application No.: US15118508Application Date: 2015-02-19
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Publication No.: US10093938B2Publication Date: 2018-10-09
- Inventor: Shigehito Ikushima , Jef Boeke
- Applicant: The Johns Hopkins University
- Applicant Address: US MD Baltimore
- Assignee: The Johns Hopkins University
- Current Assignee: The Johns Hopkins University
- Current Assignee Address: US MD Baltimore
- Agency: Johns Hopkins Technology Ventures
- International Application: PCT/US2015/016619 WO 20150219
- International Announcement: WO2015/175074 WO 20151119
- Main IPC: C12N15/81
- IPC: C12N15/81 ; C12N15/63

Abstract:
A system for controlling gene expression in yeast comprises a repressible gene expression plasmid that has a regulator binding sequence for camR and a target gene sequence. The system also includes a transcription enhancer expression plasmid; wherein said transcriptional activator protein binds to the regulator binding sequence in the absence of a transcriptional inhibitor. The system is used in a method for controlling expression of the target gene through the use of camphor. The target gene is expressed in the absence of camphor but unexpressed if camphor is added to a solution of cells containing the plasmids.
Public/Granted literature
- US20170051294A1 REGULATED SWITCH FOR GENE EXPRESSION Public/Granted day:2017-02-23
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