The present invention provides a connection structure of a plug-in power supply and a light source plate, and it relates to the field of electronic circuit connection. The connection structure of the plug-in power supply and the light source plate comprises: a card slot, which is soldered on the light source plate; a card, which is matched with the card slot and soldered on the power supply; metal contacts, which is arranged in the card slot; pre-printed circuits connected to the metal contacts and arranged on the card; the power supply is connected to the light source plate through the matching of the card slot and the card, which facilitates the disassembly and assembly and improves the production efficiency.