The present invention provides a repair structure of a line defect of an AMOLED display panel and a repair method. The conductive film (410) correspondingly overlaps and covers above the test TFT (310) and is insulated from the test TFT (310), and the repair line (420) is insulated and crossed with all the signal fanout lines (200) and the corresponding test line (330). It is realized that the repair line is directly grafted on the AMOLED display panel detecting circuit, which can utilize the present detecting circuit layout of the AMOLED display panel capable of introducing the repair line for having the repair function and saving the layout space, and has no additional requirement to the control IC, and particularly, can be applicable for the line defect repair of the small size, high resolution AMOLED display panel.