Methods, systems, and computer readable media for testing network function virtualization (NFV) are disclosed. According to one method, the method occurs at a network test controller implemented using at least one processor. The method includes determining, using network configuration information, a first insertion point for inserting a first network function tester (NFT) into a service chain comprising a plurality of virtualized network functions (VNFs). The method also includes configuring the first NFT to analyze or ignore traffic matching filtering information, wherein the traffic traverses the first NFT from at least one VNF of the plurality of VNFs. The method further includes inserting, at the first insertion point, the first NFT into the service chain.