The present invention provides a cell evaluation device, a cell evaluation method and a non-transitory computer readable recording medium storing a cell evaluation program capable of evaluating an evaluation target cell. The cell evaluation device includes an image acquisition unit that acquires a cell image obtained by imaging a cell group; a cell evaluation unit that specifies an evaluation target cell and peripheral cells around the evaluation target cell in the cell group, and evaluates the evaluation target cell based on evaluation results of the peripheral cells; and a boundary setting unit that sets a boundary in the cell image based on a state of the cell group, in which when specifying the peripheral cells, the cell evaluation unit specifies only cells that are present in a divided region where the evaluation target cell is present among plural divided regions divided by the boundary, as the peripheral cells.