Invention Grant
- Patent Title: Microparticles having reference markers arranged in different concentrations
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Application No.: US15033272Application Date: 2014-11-03
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Publication No.: US10168271B2Publication Date: 2019-01-01
- Inventor: Daniel Irimia , Ki Wan Bong
- Applicant: The General Hospital Corporation
- Applicant Address: US MA Boston
- Assignee: The General Hospital Corporation
- Current Assignee: The General Hospital Corporation
- Current Assignee Address: US MA Boston
- Agency: Fish & Richardson P.C.
- International Application: PCT/US2014/063730 WO 20141103
- International Announcement: WO2015/066635 WO 20150507
- Main IPC: G01N15/14
- IPC: G01N15/14 ; G01N33/543 ; B01L3/00 ; G01N33/569 ; G01N33/574 ; G01N15/10

Abstract:
Methods for sorting cells include: arranging microparticles into an array on a substrate in a microfluidic device, in which the microparticles each include multiple reference markers; introducing multiple cells to the array of microparticles under conditions that enable at least some of the cells to adhere to the microparticles; removing the microparticles, to which the cells are adhered, from the substrate; transferring the microparticles, to which the cells are adhered, to a detection region; and detecting, for each of two or more microparticles that pass through the detection region, a microparticle feature; and sorting the two or more microparticles based on the detected features, in which the detected features are related to a phenotype of the cells.
Public/Granted literature
- US20160282255A1 CELL SORTING Public/Granted day:2016-09-29
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