A bidirectional scanning unit, a driving method and a gate driving circuit are provided. The bidirectional scanning unit includes a first-stage sub unit and a second-stage sub unit, the bidirectional scanning unit can output a scanning signal stage by stage in a direction from the first-stage sub unit to the second-stage sub unit, and can also output a scanning signal stage by stage in a direction from the second-stage sub unit to the first-stage sub unit. The first-stage sub unit coordinates with the second-stage sub unit in a scanning process, when one of the first-stage sub unit and the second-stage sub unit outputs the scanning signal, the other of the first-stage sub unit and the second-stage sub unit does not output the scanning signal.