Live migration may be performed for virtual computing resources utilizing network-based storage. A virtual compute instance operating at a source host may be moved to a destination host. The virtual compute instance may be a client of a network-based storage resource that stores data for the virtual compute instance. Access to the data stored for the virtual compute instance may be limited to the source host. When migration is performed, the destination host may be prepared to assume operation of the virtual compute instance. Operation of the virtual compute instance at the source host may be paused and the access to the data at the network-based storage resource may be modified to limit access to the destination host. Operation of the virtual compute instance may then resume at the destination host.