- Patent Title: Transgenic animal for production of antibodies having minimal CDRS
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Application No.: US15996373Application Date: 2018-06-01
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Publication No.: US10172334B2Publication Date: 2019-01-08
- Inventor: William Don Harriman , Robert Etches , Philip A. Leighton
- Applicant: Crystal Bioscience Inc.
- Applicant Address: US CA San Diego
- Assignee: Crystal Bioscience Inc.
- Current Assignee: Crystal Bioscience Inc.
- Current Assignee Address: US CA San Diego
- Agency: Bozicevic, Field & Francis LLP
- Agent James S. Keddie
- Main IPC: A01K67/00
- IPC: A01K67/00 ; A01K67/027 ; C12P21/02 ; C12N15/87 ; C12N15/85 ; C07K16/00

Abstract:
A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.
Public/Granted literature
- US20180338479A1 TRANSGENIC ANIMAL FOR PRODUCTION OF ANTIBODIES HAVING MINIMAL CDRS Public/Granted day:2018-11-29
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