Invention Grant
- Patent Title: Microorganisms engineered to produce phenol and its derivatives
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Application No.: US14908967Application Date: 2014-08-12
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Publication No.: US10174346B2Publication Date: 2019-01-08
- Inventor: David Nielsen , Shawn Pugh , Brian Thompson
- Applicant: David Nielsen , Shawn Pugh , Brian Thompson
- Applicant Address: US AZ Scottsdale
- Assignee: Arizona Board of Regents on behalf of Arizona State University
- Current Assignee: Arizona Board of Regents on behalf of Arizona State University
- Current Assignee Address: US AZ Scottsdale
- Agency: Quarles & Brady LLP
- Agent Gavin J. Milczarek-Desai
- International Application: PCT/US2014/050756 WO 20140812
- International Announcement: WO2015/031048 WO 20150305
- Main IPC: C12P1/04
- IPC: C12P1/04 ; C12P5/00 ; C12P17/00 ; C12P7/22 ; C12N9/88 ; C12N9/90 ; C12P7/44

Abstract:
Novel methods for the in vivo production of phenol from renewable substrates using a recombinant microorganism (FIG. 1). Additionally, methods for the in vivo production of catechol and cis,cis-muconic acid from renewable substrates using a recombinant microorganism are disclosed. A host cell expresses at least one gene encoding a polypeptide that possesses isochorismate synthase activity, at least one gene encoding a polypeptide that possesses isochorismate pyruvate lyase activity, and at least one gene encoding a polypeptide that possesses salicylic acid decarboxylase activity. In the case of catechol, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxygenase activity. In the case of cis,cis-muconic acid, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxygenase activity and at least one gene encoding a polypeptide that possesses catechol-1,2-dioxygenase activity.
Public/Granted literature
- US20160160244A1 Microorganisms Engineered to Produce Phenol and Its Derivatives Public/Granted day:2016-06-09
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