A method, device and multipoint control unit device for adjusting and controlling a sub-picture in multiple pictures are provided. The method includes: acquiring the N original video images sent from a plurality of video terminals, adjusting the resolutions of the N original video images and correspondingly displaying the N resolution-adjusted video images in N regions on the display screen of the multipoint control unit, wherein N is a natural number equal to or greater than 1; performing a resolution increase adjustment processing on the video image in any one of the N regions to acquire a resolution-increased video image; and cutting, according to the information of the region where the resolution-increased video image is located, the resolution-increased video image to obtain an image which is located in the region where the resolution-increased video image is located after the cutting. According to the solutions provided herein, an image of interest can be increased in resolution and moved in the region where the image of interest is located on the premise of not affecting the video images in the other regions.