The present invention relates to Acanthophysium sp. KMF001 strain having high cellulose activity, and more particularly, to a novel Acanthophysium sp. KMF001 strain having the ability to produce endo-β-1,4-glucanase, β-glucosidase, and cellobiohydrolase and to a culture of the strain. The novel Acanthophysium sp. KMF001 strain according to the present invention can produce highly active cellulases, including endo-β-1,4-glucanase, β-glucosidase, and cellobiohydrolase, and thus can be advantageously used not only for enzymatic saccharification of lignocellulosic biomass, but also in various industrial fields, including the pulp and paper making industry, the detergent industry, the agricultural product processing industry, the fiber industry, and the livestock industry, in which the degradation of cellulose is required.