An automatic slicing and collecting device, the device including: a first tank, a vibrating microtome, a first pipeline, and a second pipeline. The first tank is filled with a buffer solution. The vibrating microtome is disposed in the first tank. One end of the first pipeline is connected to the vibrating microtome to collect sections, and the other end of the first pipeline is connected to a pump. The pump includes a reversible motor. The first pipeline is provided with a first valve, and a filter is disposed between the pump and the first pipeline. One end of the second pipeline is disposed between the first valve of the first pipeline and the pump, and the second pipeline is provided with a second valve.