The present application discloses a rubbing roller for rubbing an alignment layer of a liquid crystal display substrate, including a rotary roller and a rubbing cloth substantially surrounding a circumferential surface of the rotary roller. The rubbing cloth includes at least a first portion and a second portion, each having a width substantially corresponding to a length of the rotary roller and a length corresponding to a division of a circumference of the rotary roller. Substantially all fibers of the first portion extending away from the rotary roller and leaning towards a first end of the rotary roller, and substantially all fibers of the second portion extending away from the rotary roller and leaning towards a second end of the rotary roller.