Invention Grant
- Patent Title: Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
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Application No.: US14215406Application Date: 2014-03-17
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Publication No.: US10202630B2Publication Date: 2019-02-12
- Inventor: Gary Lesnicki , Mark Young
- Applicant: ALDERBIO HOLDINGS LLC
- Applicant Address: US NV Las Vegas
- Assignee: ALDERBIO HOLDINGS LLC
- Current Assignee: ALDERBIO HOLDINGS LLC
- Current Assignee Address: US NV Las Vegas
- Agency: LeClairRyan PLLC
- Agent Robin L. Teskin
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C07K16/00

Abstract:
Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.
Public/Granted literature
- US20140287459A1 TEMPERATURE SHIFT FOR HIGH YIELD EXPRESSION OF POLYPEPTIDES IN YEAST AND OTHER TRANSFORMED CELLS Public/Granted day:2014-09-25
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