Invention Grant
- Patent Title: SRM/MRM assay for the tyrosine-protein kinase receptor UFO (AXL) protein
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Application No.: US15307753Application Date: 2015-04-30
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Publication No.: US10203336B2Publication Date: 2019-02-12
- Inventor: David B. Krizman , Todd Hembrough , Adele Blackler , Wei-Li Liao
- Applicant: Expression Pathology, Inc.
- Applicant Address: US MD Rockville
- Assignee: Expression Pathology, Inc.
- Current Assignee: Expression Pathology, Inc.
- Current Assignee Address: US MD Rockville
- Agency: Edell, Shapiro & Finnan, LLC
- International Application: PCT/US2015/028667 WO 20150430
- International Announcement: WO2015/168484 WO 20151105
- Main IPC: G01N33/68
- IPC: G01N33/68 ; G01N33/574

Abstract:
Peptides from the tyrosine-protein kinase receptor UFO protein (AXL) are provided that are particularly advantageous for quantifying the AXL protein directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed and include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein digest is prepared from the biological sample and the AXL protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described.
Public/Granted literature
- US20170052196A1 SRM/MRM Assay for the tyrosine-protein kinase receptor UFO (AXL) protein Public/Granted day:2017-02-23
Information query
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